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KMID : 0613820200300121101
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Journal of Life Science
2020 Volume.30 No. 12 p.1101 ~ p.1108
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Optimization of the Conditions of Flavonoid Extraction From Tartary Buckwheat Sprout Using Response Surface Methodology
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Shin Ji-Young
Choi I-Seul
Hwang Jin-Woo
Yang Jun-Ho
Lee Yoon-Hyeong
Kim So-I
Cha Eun-Ji
Yang Ji-Young
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Abstract
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Tartary buckwheat is a grain with many flavonoids, such as rutin, quercetin, kaempferol, and myricetin. This study aimed to optimize extraction conditions to maximize the rutin, quercetin, and myricetin contents of tartary buckwheat sprout extracts using response surface methodology. A Box- Behnken design containing 15 experiments was employed to evaluate the effects of extraction conditions, such as temperature (X1, 50~70℃), extraction time (X2, 5~9 hr), and ethanol concentration (X3, 60~90%). The coefficients of determination (R2) for all the dependent variables (extraction temperature, extraction time, and extraction ethanol concentration) were determined to be over 0.95, indicating significance. The p-value of the model in lack of fit was over 0.1 than means, indicating that the model was well predicted. The optimal extraction conditions for rutin, quercetin, and myricetin contents were obtained at X1=51.03, X2=6.62, and X3=69.16, respectively. Under these optimal conditions, the predicted rutin, quercetin, and myricetin contents were 808.467 μg/ml, 193.296 μg/ml, and 37.360 μg/ ml, respectively. For the validation of the model, ten experiments were performed and the experimental rutin and quercetin contents were measured at 802.84±8.49 μg/ml, 193.76±2.80 μg/ml, and 34.84±0.43 μg/ml, respectively. The experimental rutin and quercetin contents were similar to the predicted contents, but the experimental myricetin content was lower than predicted.
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KEYWORD
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Myricetin, quercetin, response surface methodology, rutin, tartary buckwheat sprout
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